Hangzhou Environment Research Institute of CCTEG,Hangzhou ,China

为了培养提高煤气化废水可生化性的厌氧菌,采用选择性传代培养及"共代谢基质-目标污染物"双底物模式对厌氧菌群进行了高酚高氨氮耐受驯化培养,研究了水温、pH等培养条件对菌群培养的影响,并进行了最优条件下厌氧菌处理煤气化废水试验。试验结果表明:随着培养时间的延长和底物初始浓度的提高,厌氧菌群逐渐适应了高酚高氨氮水质环境。厌氧菌群的最佳生长温度为30℃,最佳生长pH为8。在最佳温度及pH条件下,接种10%驯化后的菌液至COD质量浓度1 580 mg/L、总酚质量浓度422 mg/L、氨氮浓度139 mg/L的煤气化废水中,添加20 mg/L邻苯二酚作为共代谢基质,经过20 d的培养,COD、总酚平均去除率分别达到了40.25%、41.38%,煤气化废水可生化性B/C由0.21提高至0.45。

In order to cultivate the anaerobic bacteria which can enhance the biodegradability of gasification wastewater,the paper domesticated and cultured the anaerobic bacteria with high phenol and high ammonia nitrogen tolerant by selective subculture and “Co metabolism matrix-Target pollutant” double substrate mode.The paper studied the effects of culture conditions such as temperature and pH on the culture of bacteria.The anaerobic bacteria were used to treat coal gasification wastewater under the optimal conditions.The results showed that with the prolongation of culture time and the increase of substrate initial concentration,anaerobic bacteria adapted to the high phenol and high ammonia nitrogen gradually.The optimum growth temperature of anaerobic bacteria was 30 ℃ and the optimum pH for growth was 8.Under optimum temperature and pH conditions,10% acclimated bacteria were inoculated into coal gasification wastewater with COD concentration 1 580 mg/L,total phenol concentration 422 mg/L and ammonia nitrogen concentration 139 mg/L.20 mg/L catechol was added as the co-metabolism matrix.After 20 d culture,the average removal rate of COD and total phenol reached 40.25% and 41.38% respectively.The B/C from coal gasification wastewater was increased from 0.21 to 0.45.

中国煤炭科工集团有限公司科技创新创业资金专项资助项目(2018QN012)；