Hebei Province Ecology Environmental Monitoring Center
China South to North Water Diversion Group Middle Route Corporartion Ltd., Hebei Brancht
Shijiazhuang Hi-tech Industrial Development Zone Water Supply and Drainage company

采用酶底物法和荧光光度法对水源水、景观水、污水中粪大肠菌群进行检测。通过检测高浓度标准样品与低浓度标准样品发现,酶底物法和荧光光度法相比，荧光光度法检测结果相对误差更小，跟接近于真值，其结果更为准确。荧光光度法相对标准偏差低于酶底物法，表明荧光光度法检测的稳定性优于酶底物法。通过选取不同类型的实际样品分别用2种方法测定，并对结果进行统计学分析，发现2种方法在统计学中具有相关性，无显著差异。采用荧光光度法测定粪大肠菌群具有测定时间短，减少了人为误差，同时避免了测定时间对结果的影响，特别适合应急检测。

Enzyme substrate method and fluorescence spectrophotometry were used to detect fecal coliform group in source water, landscape water and sewage. Compared with the method of enzyme substrate and fluorophotometry, the result of fluorophotometry is less relative error, closer to the true value and more accurate. The relative standard deviation of fluorometric method was lower than that of enzyme substrate method, indicating that the stability of fluorometric method was better than that of enzyme substrate method. Two methods were used to determine different types of samples, and the results were statistically analyzed. It was found that the two methods had correlation in statistics, and there was no significant difference. The fluorometric method can be used to determine fecal coliform group in a short time, reduce the human error and avoid the influence of the measuring time on the results, especially suitable for emergency detection.

张 茵1，刘溪南2,王 静3，刘 娟3. 水中粪大肠菌群的测定-酶底物法与荧光光度法的比较[J]. 煤炭与化工, 2024, 47(3): 151- 154，160..

Zhang Yin1，Liu Xinan2，Wang Jing3，Liu Juan3. Determination of fecal coliform group in water——comparison of enzyme substrate method and fluorescence spectrophotometry. CCI, 2024, 47(3): 151- 154，160..